Validating transcripts with probes and imaging technology

Although, the information for protein synthesis is stored in DNA, however, for achieving successful gene expression several key classes of RNA molecules works in coordination.

Three kinds of RNAs, messenger RNA (m RNA), transfer RNA (t RNA) & ribosomal RNA (r RNA) carry out their specific tasks, leading the accurate synthesis of proteins, which is critical to the proper functioning of cells and organisms.

Always pull the pipette away from the sample port before releasing the plunger, instead of releasing the plunger immediately.

The microfluidic channels are a closed system, and doing so will withdraw the sample back into the tip.

If you need to upgrade your instrument software to version 2.0 or later, please contact [email protected]

Additional maintenance tasks may need to be performed as necessary to ensure proper operation of the instrument.

Another technology is fluorescence in situ hybridization (FISH), wherein nucleic acids are targeted by fluorescently labeled probes and then visualized via microscopy; this technology is an extension of methods that once used radioactive probes and autoradiography but have since been adapted to use nonradioactive labels (5–11).

If your SPRINT has software version 2.0 or later, you can redefine the associated RLF with completed runs from your instrument.These live-cell imaging studies have dramatically improved our understanding of the role of RNA dynamics in various cellular functions.Nevertheless, a range of newer experimental approaches and/or protocols in RNA imaging technologies are continuously evolving.Annotation of herpesvirus genomes has traditionally been undertaken through the detection of open reading frames and other genomic motifs, supplemented with sequencing of individual c DNAs.Second generation sequencing and high-density microarray studies have revealed vastly greater herpesvirus transcriptome complexity than is captured by existing annotation.